Mubashir, S. S., Khan, N. A., Padder, B. A., Bhat, Z. A. & Bhat, S. N. Morphological differentiation of Venturia species infecting different pome and stone fruits in Jammu and Kashmir, India. Indian Phytopath. 77, 335–343 (2024).
Google Scholar
Mubashir, S. S. et al. Baseline sensitivities of Venturia inaequalis populations to the Difenaconazole, a sterol demethylation inhibitor fungicide. Front. Crop Improv. 11, 2965–2968 (2023).
Google Scholar
Köller, W., Wilcox, W., Barnard, J., Jones, A. & Braun, P. Detection and quantification of resistance of Venturia inaequalis populations to sterol demethylation inhibitors. Phytopathology 87, 184–190 (1997).
Google Scholar
Nabi, A. et al. First report of Myclobutanil resistance and shift in sensitivity to difenoconazole and flusilazole in North-western Himalyan Venturia inaequalis populations. Australas. Plant Pathol. 52, 13–22 (2023).
Google Scholar
Carisse, O. & Jobin, T. Resistance to dodine in populations of Venturia inaequalis in Quebec, Canada. Plant. Health Progress. 11, 17 (2010).
Google Scholar
Polat, Z. & Bayraktar, H. Resistance of Venturia inaequalis to multiple fungicides in Turkish apple orchards. J. Phytopathol. 169, 360–368 (2021).
Google Scholar
Gao, L., Berrie, A., Yang, J. & Xu, X. Within-and between‐orchard variability in the sensitivity of Venturia inaequalis to myclobutanil, a DMI fungicide, in the UK. Pest Manage. Science: Former. Pesticide Sci. 65, 1241–1249 (2009).
Google Scholar
Hoffmeister, M., Zito, R., Böhm, J. & Stammler, G. Mutations in Cyp51 of Venturia inaequalis and their effects on DMI sensitivity. J. Plant Dis. Prot. 128, 1467–1478 (2021).
Google Scholar
Chatzidimopoulos, M., Zambounis, A., Lioliopoulou, F. & Vellios, E. Detection of Venturia inaequalis isolates with multiple resistance in Greece. Microorganisms 10, 2354 (2022).
Google Scholar
Yaegashi, H., Hirayama, K., Akahira, T. & Ito, T. Point mutation in CYP51A1 of Venturia inaequalis is associated with low sensitivity to sterol demethylation inhibitors. J. Gen. Plant Pathol. 86, 245–249 (2020).
Google Scholar
Chartrain, L. & Brown, J. K. Molecular evolution and mechanisms of fungicide resistance in plant pathogenic fungi. In Burleigh Dodds Series in Agricultural Science (ed. Ferreira, J. B.) 1–37 (Burleigh Dodds Science Publishing Limited Cambridge, UK 2023).
Dooley, H. Fungicide-resistance Management Tactics: Impacts on Zymoseptoria Tritici Populations (University of Reading, 2015).
Google Scholar
Köller, W., Parker, D., Turechek, W., Avila-Adame, C. & Cronshaw, K. A two-phase resistance response of Venturia inaequalis populations to the QoI fungicides kresoxim-methyl and trifloxystrobin. Plant. Dis. 88, 537–544 (2004).
Google Scholar
Prencipe, S., Sillo, F., Garibaldi, A., Gullino, M. L. & Spadaro, D. Development of a sensitive TaqMan qPCR assay for detection and quantification of Venturia inaequalis in apple leaves and fruit and in air samples. Plant. Dis. 104, 2851–2859 (2020).
Google Scholar
Villani, S. M., Hulvey, J., Hily, J. M. & Cox, K. D. Overexpression of the CYP51A1 gene and repeated elements are associated with differential sensitivity to DMI fungicides in Venturia Inaequalis. Phytopathology 106, 562–571 (2016).
Google Scholar
Standish, J. R., Brenneman, T. B., Brewer, M. T. & Stevenson, K. L. Assessing fitness costs and phenotypic instability of fentin hydroxide and tebuconazole resistance in Venturia Effusa. Plant. Dis. 103, 2271–2276 (2019).
Google Scholar
Chen, F. et al. Baseline sensitivity of Monilinia fructicola from China to the DMI fungicide SYP-Z048 and analysis of DMI-resistant mutants. Plant. Dis. 96, 416–422 (2012).
Google Scholar
Zwiers, L. H., Stergiopoulos, I., Gielkens, M. M., Goodall, S. D. & De Waard, M. A. ABC transporters of the wheat pathogen Mycosphaerella graminicola function as protectants against biotic and xenobiotic toxic compounds. Mol. Genet. Genomics. 269, 499–507 (2003).
Google Scholar
Hawkins, N. & Fraaije, B. Fitness penalties in the evolution of fungicide resistance. Annu. Rev. Phytopathol. 56, 339–360 (2018).
Google Scholar
Mikaberidze, A. & McDonald, B. A. Fitness cost of resistance: impact on management. Fungicide resistance in plant pathogens: principles and a guide to practical management, 77–89 (2015).
Brent, K. J. & Hollomon, D. W. Fungicide Resistance: The Assessment of riskVol. 2 (Global Crop Protection Federation Brussels, 1998).
Google Scholar
Cools, H. J. & Fraaije, B. A. Update on mechanisms of azole resistance in Mycosphaerella graminicola and implications for future control. Pest Manage. Sci. 69, 150–155 (2013).
Google Scholar
Gisi, U. Assessment of selection and resistance risk for demethylation inhibitor fungicides in aspergillus fumigatus in agriculture and medicine: a critical review. Pest Manage. Sci. 70, 352–364 (2014).
Google Scholar
Frederick, Z. A., Villani, S. M. & Cox, K. D. The effect of delayed-dormant chemical treatments on demethylation inhibitor (DMI) sensitivity in a DMI-resistant population of Venturia Inaequalis. Plant. Dis. 99, 1751–1756 (2015).
Google Scholar
Lucas, J. A., Hawkins, N. J. & Fraaije, B. A. The evolution of fungicide resistance. Adv. Appl. Microbiol. 90, 29–92 (2015).
Google Scholar
Nassreen, F. Assessment of Resistance Development in Venturia inaequalis (Cke.) Wint. And Alternaria Mali Roberts against Systemic Fungitoxicants in Kashmir Valley (SKUAST-K, 2008).
Kacho, N. F., Banday, S. & Ashraf, S. Venturia inaequalis sensitivity to ergosterol biosynthesis inhibitors in Kashmir valley. Indian Phytopath. 66, 284–286 (2013).
Schnabel, G. & Jones, A. L. The 14α-demethylasse (CYP51A1) gene is overexpressed in Venturia inaequalis strains resistant to myclobutanil. Phytopathology 91, 102–110 (2001).
Google Scholar
Nene, Y. & Thapilyal, L. Poisoned food technique of fungicides in plant disease control. (2002).
Murray, M. & Thompson, W. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8, 4321–4326 (1980).
Google Scholar
Chapman, K. S., Sundin, G. W. & Beckerman, J. L. Identification of resistance to multiple fungicides in field populations of Venturia Inaequalis. Plant. Dis. 95, 921–926 (2011).
Google Scholar
Stević, M., Vukša, P. & Elezović, I. Resistance of Venturia inaequalis to demethylation inhibiting (DMI) fungicides. Žemdirbyste 97, 65–72 (2010).
Villani, S. M., Biggs, A. R., Cooley, D. R., Raes, J. J. & Cox, K. D. Prevalence of myclobutanil resistance and difenoconazole insensitivity in populations of Venturia Inaequalis. Plant. Dis. 99, 1526–1536 (2015).
Google Scholar
Köller, W., Parker, D. & Reynolds, K. Baseline sensitivities of Venturia inaequalis to sterol demethylation inhibitors. (1991).
Smith, F. D., Parker, D. M. & Köller, W. Sensitivity distribution of Venturia inaequalis to the sterol demethylation inhibitor flusilazole: baseline sensitivity and implications for resistance monitoring. Phytopathology 81, 392–396 (1991).
Google Scholar
Limon, C. L. Genetics behind the Variability in Sensitivity to the Demethylation Inhibitor (DMI) Fungicides Myclobutanil and Tebuconazole in Venturia Inaequalis (University of Reading, 2018).
Google Scholar
Cañas-Gutiérrez, G. P. et al. Analysis of the CYP51 gene and encoded protein in propiconazole‐resistant isolates of Mycosphaerella fijiensis. Pest Manage. Science: Former. Pesticide Sci. 65, 892–899 (2009).
Google Scholar
Becher, R. & Wirsel, S. G. Fungal cytochrome P450 sterol 14α-demethylase (CYP51) and azole resistance in plant and human pathogens. Appl. Microbiol. Biotechnol. 95, 825–840 (2012).
Google Scholar
Lichtemberg, P. S. et al. The point mutation G461S in the MfCYP51 gene is associated with tebuconazole resistance in Monilinia fructicola populations in Brazil. Phytopathology 107, 1507–1514 (2017).
Google Scholar
Parker, J. E. et al. Resistance to antifungals that target CYP51. J. Chem. Biol. 7, 143–161 (2014).
Google Scholar
Warrilow, A. G. et al. The evolution of azole resistance in Candida albicans sterol 14α-demethylase (CYP51) through incremental amino acid substitutions. Antimicrob. Agents Chemother. 63, 101128aac02586–101128aac02518 (2019).
Google Scholar
Morio, F., Loge, C., Besse, B., Hennequin, C. & Le Pape, P. Screening for amino acid substitutions in the Candida albicans Erg11 protein of azole-susceptible and azole-resistant clinical isolates: new substitutions and a review of the literature. Diagn. Microbiol. Infect. Dis. 66, 373–384 (2010).
Google Scholar
Zhan, J., Stefanato, F. & McDonald, B. A. Selection for increased cyproconazole tolerance in Mycosphaerella graminicola through local adaptation and in response to host resistance. Mol. Plant. Pathol. 7, 259–268 (2006).
Google Scholar
Leroux, P., Albertini, C., Gautier, A., Gredt, M. & Walker, A. S. Mutations in the CYP51 gene correlated with changes in sensitivity to sterol 14α-demethylation inhibitors in field isolates of Mycosphaerella Graminicola. Pest Manage. Science: Former. Pesticide Sci. 63, 688–698 (2007).
Google Scholar
Stammler, G. et al. Frequency of different CYP51-haplotypes of Mycosphaerella graminicola and their impact on epoxiconazole-sensitivity and-field efficacy. Crop Protect. 27, 1448–1456 (2008).
Google Scholar
Mair, W. et al. Proposal for a unified nomenclature for target-site mutations associated with resistance to fungicides. Pest Manage. Sci. 72, 1449–1459 (2016).
Google Scholar
Delye, C., Laigret, F. & Corio-Costet, M. F. A mutation in the 14 alpha-demethylase gene of Uncinula necator that correlates with resistance to a sterol biosynthesis inhibitor. Appl. Environ. Microbiol. 63, 2966–2970 (1997).
Google Scholar
Tucker, M. A. et al. Analysis of mutations in West Australian populations of Blumeria graminis f. sp. hordei CYP51 conferring resistance to DMI fungicides. Pest Manage. Sci. 76, 1265–1272 (2020).
Google Scholar
Karaoglanidis, G., Thanassoulopoulos, C. C. & Ioannidis, P. Fitness of Cercospora Beticola field isolates–resistant and–sensitive to demethylation inhibitor fungicides. Eur. J. Plant. Pathol. 107, 337–347 (2001).
Google Scholar
Karaoglanidis, G. & Thanassoulopoulos, C. Phenotypic instability of Cercospora Beticola Sacc. Strains expressing resistance to the sterol demethylation-inhibiting (DMI) fungicide flutriafol after cold exposure. J. Phytopathol. 150, 692–696 (2002).
Google Scholar
Köller, W., Smith, F. & Reynolds, K. Phenotypic instability of flusilazole sensitivity in Venturia Inaequalis. Plant. Pathol. 40, 608–611 (1991).
Google Scholar
Cox, K., Bryson, P. & Schnabel, G. Instability of propiconazole resistance and fitness in Monilinia fructicola. Phytopathology 97, 448–453 (2007).
Google Scholar